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Image Search Results


Highlights

Journal: Neuron

Article Title: Mutant Huntingtin Disrupts the Nuclear Pore Complex

doi: 10.1016/j.neuron.2017.03.023

Figure Lengend Snippet: Highlights

Article Snippet: Troy Littleton https://biology.mit.edu/people/j_troy_littleton Recombinant DNA Construct: 6xStop-(CAG)80 Laboratory of Dr. Laura Ranum; ( Bañez-Coronel et al., 2015 ) N/A Constructs: Full-length HTT 22Q and 82Q Laboratory of Dr. Christopher Ross; ( Ratovitski et al., 2015 ) N/A Software and Algorithms Fiji Software Fiji Contributors http://fiji.sc/ RRID: SCR_002285 Imaris Bitplane http://www.bitplane.com/ RRID: SCR_007370 Volocity 3D Image Analysis Software Perkin-Elmer http://www.perkinelmer.com/pages/020/cellularimaging/products/volocity.xhtml RRID: SCR_002668 Scion Image Analysis Software Scion Corporation http://www.scioncorp.com/pages/product_prices.htm#Software RRID: SCR_008673 Axiovision Imaging Software Carl Zeiss http://www.zeiss.com/microscopy/en_de/products/microscope-software/axiovision-for-biology.html RRID:SCR_002677 ZEN Digital Imaging for LSM 800 Carl Zeiss http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen.html#introduction RRID: SCR_013672 GraphPad Prism 7 GraphPad Software http://www.graphpad.com/ RRID: SCR_002798 Other Lipofectamine 2000 Thermo Fisher Scientific Cat#11668027 SuperBlock Blocking Buffer Thermo Fisher Scientific Cat#37515 Protein Block Serum-Free Dako Cat#X0909 Antibody Diluent with Background Reducing Components Dako Cat#S3022 HC-TekTM Epitope Retrieval Solution IHC World Cat#IW-1100 Zeiss Axiovert 100 Inverted Microscope Carl Zeiss N/A Zeiss LSM 800 Microscope Carl Zeiss N/A Zeiss Apotome Carl Zeiss N/A Open in a separate window Highlights HD human and mouse brain share profound nuclear pore complex pathology HD iPS neurons mirror dysregulated HD human brain nuclear pore complex pathology Aberrant nucleocytoplasmic transport is identified in various in vitro HD models HD neurotoxicity can be mitigated with rescue of nucleocytoplasmic transport defects

Techniques: Plasmid Preparation, Control, Recombinant, Viability Assay, Dominant Negative Mutation, Construct, Software, Imaging, Blocking Assay, Inverted Microscopy, Microscopy